Association and linkage studies between bipolar affective disorder and the polymorphic CAG/CTG repeat loci ERDA1, SEF2-1B, MAB21L and KCNN3

Several reports have suggested the presence of anticipation in bipolar affective disorder (BPAD). in addition, independent studies using the RED (repeat expansion detection) have shown association between BPAD and longer CAG/CTG repeats. Therefore loci with large CAG/CTG repeats are plausible candidates in the inheritance of BPAD. the present study assesses the length of the repeats in four loci: the ERDA-1 locus which is known to account for most of the long CAG repeats detected by RED, the SEF2-1b locus which is placed in a region where positive linkage results have been reported and the loci MAB21L and KCNN3 as functional candidate genes. A Brazilian case-control sample with 115 unrelated BPAD patients and 196 healthy control subjects and 14 multiply affected bipolar families was investigated. With the case-control design the distribution of alleles between the two groups did not approach statistical significance. the extended transmission disequilibrium test (ETDT) performed in our families did not show evidence for linkage disequilibrium. Parametric and non-parametric linkage analysis also did not provide support for linkage between any of the four loci and BPAD. Our data do not support the hypothesis that variation at the polymorphic CAG/CTG repeat loci ERDA-1, SEF2-1b, MAB21L or KCNN3 influence susceptibility to BPAD in our sample.Univ São Paulo, Sch Med, Inst Psychiat, Neurosci Lab LIM 27, BR-05403010 São Paulo, BrazilUniv São Paulo, Sch Med, Heart Inst InCor, Dept Med,Lab Genet & Mol Cardiol LIM 13, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Psychiat, São Paulo, BrazilInst Psychiat, Div Psychol Med, Sect Genet Epidemiol & Biostat, London, EnglandUniversidade Federal de São Paulo, Dept Psychiat, São Paulo, BrazilWeb of Scienc

Amerindian (but not African or European) ancestry is significantly associated with diurnal preference within an admixed Brazilian population

Significant questions remain unanswered regarding the genetic versus environmental contributions to racial/ethnic differences in sleep and circadian rhythms. We addressed this question by investigating the association between diurnal preference, using the morningness–eveningness questionnaire (MEQ), and genetic ancestry within the Baependi Heart Study cohort, a highly admixed Brazilian population based in a rural town. Analysis was performed using measures of ancestry, using the Admixture program, and MEQ from 1,453 individuals. We found an association between the degree of Amerindian (but not European of African) ancestry and morningness, equating to 0.16 units for each additional percent of Amerindian ancestry, after adjustment for age, sex, education, and residential zone. To our knowledge, this is the first published report identifying an association between genetic ancestry and MEQ, and above all, the first one based on ancestral contributions within individuals living in the same community. This previously unknown ancestral dimension of diurnal preference suggests a stratification between racial/ethnic groups in an as yet unknown number of genetic polymorphisms

Consumption of fruits and vegetables in a group of valencian children of school age.

Introducción: En los últimos 25 años ha habido un gran cambio en los hábitos alimentarios en España. Objetivos: Calcular y valorar el consumo de frutas, verduras y hortalizas en niños de edad escolar e identificar el tipo de postre que toman analizando la influencia de los niños en la compra de fruta y verdura que realizan los padres. Material y métodos: Se realizó un cuestionario dirigido a los padres de niños y niñas en edad escolar que estaban cursando primaria. De 155 cuestionarios entregados en el colegio, se obtuvo una muestra de 116. Resultados: Solo el 5,17% de los niños consumen 3 ó más raciones de frutas al día. El 30,17% consumen 1 ó 2 raciones de verduras y hortalizas al día. El 18,97% toman fruta como postre de forma habitual. El 25% de los padres compran fruta la mayoría de los días porque sus hijos se la piden y el 12,93% compran verdura. Discusión: No hay un consumo suficiente de frutas, verduras y hortalizas entre los escolares estudiados. Los niños influyen en la compra de frutas, verduras y hortalizas que realizan sus padres. Conclusiones: El consumo de frutas, verduras y hortalizas en la mayoría de estos niños, no sigue las recomendaciones de las Guías Dietéticas. El consumo de fruta como postre de forma habitual es bajo. Los niños tienen más influencia en la compra de frutas que en las de verduras.Introduction: In the last 25 years there has been a great change in the food habits in Spain. Objectives: To calculate and assess the consumption of fruits and vegetables in school children. To identify the type of desserts eaten and analyze the influence children have on the purchase of fruit and vegetables made by parents. Materials and methods: A survey was carried out on parents of primary school children. A total of 155 questionnaires were handed into the school, obtaining a sample of 116 completed questionnaires. Results: Only 5.17% eat 3 or more portions of fruit per day. Regarding vegetables, 30.17% consume 1 or 2 portions. 18.97% have fruit as a dessert. 25% of the parents purchase fruit most days as a result of the children request and 12.93% purchase vegetables. Discussion: Children do not consume enough fruit and vegetables. Children have an influence on fruit and vegetable purchases. Conclusions: The majority of the studied children do not follow the dietary recommendations regarding consumption of fruit and vegetables. Consumption of fruit for dessert is low. Children have a bigger influence on fruit purchases than in vegetables purchases.Nutrición humana y dietétic

Distribution and heritability of diurnal preference (chronotype) in a rural Brazilian family-based cohort, the Baependi study

Diurnal preference (chronotype) is a useful instrument for studying circadian biology in humans. It harbours trait-like dimensions relating to circadian period and sleep homeostasis, but also has ontogenetic components (morningness increases with age). We used the Morningness-Eveningness questionnaire (MEQ) in the Baependi study, a family-based cohort study based in a small town in Minas Gerais, Brazil. The population is highly admixed and has a cohesive and conservative lifestyle. 825 individuals (497 female) aged 18-89 years (average ± SD = 46.4 ± 16.3) and belonging to 112 different families participated in this study. The average MEQ score was 63.5 ± 11.2 with a significant (P < 0.0001) linear increase with age. Morningness was significantly (P < 0.0001) higher in the rural (70.2 ± 9.8) than in the municipal zone (62.6 ± 11.1), and was also significantly (P = 0.025) higher in male (64.6 ± 10.9) than in female (62.8 ± 11.2) participants. Thus, in spite of universal access to electricity, the Baependi population was strongly shifted towards morningness, particularly in the rural zone. Heritability of MEQ score was 0.48 when adjusted for sex and age, or 0.38 when adjusted for sex, age, and residential zone. The reported MEQ score heritability is more akin to those of previous twin studies than previous family studies

Flow shop rescheduling under different types of disruption

This is an Accepted Manuscript of an article published by Taylor & Francis in International Journal of Production Research on 2013, available online:http://www.tandfonline.com/10.1080/00207543.2012.666856Almost all manufacturing facilities need to use production planning and scheduling systems to increase productivity and to reduce production costs. Real-life production operations are subject to a large number of unexpected disruptions that may invalidate the original schedules. In these cases, rescheduling is essential to minimise the impact on the performance of the system. In this work we consider flow shop layouts that have seldom been studied in the rescheduling literature. We generate and employ three types of disruption that interrupt the original schedules simultaneously. We develop rescheduling algorithms to finally accomplish the twofold objective of establishing a standard framework on the one hand, and proposing rescheduling methods that seek a good trade-off between schedule quality and stability on the other.The authors would like to thank the anonymous referees for their careful and detailed comments that helped to improve the paper considerably. This work is partially financed by the Small and Medium Industry of the Generalitat Valenciana (IMPIVA) and by the European Union through the European Regional Development Fund (FEDER) inside the R + D program "Ayudas dirigidas a Institutos tecnologicos de la Red IMPIVA" during the year 2011, with project number IMDEEA/2011/142.Katragjini Prifti, K.; Vallada Regalado, E.; Ruiz García, R. (2013). Flow shop rescheduling under different types of disruption. International Journal of Production Research. 51(3):780-797. https://doi.org/10.1080/00207543.2012.666856S780797513Abumaizar, R. J., & Svestka, J. A. 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Phenotypic characterization and identification of bacterial isolates from smoked fish

This study aimed to phenotypically characterize and identify bacterial isolates from smoked fish. From the seven morphologically different bacterial colonies, four of them were identified using selective/differential media. The two isolates belong to genus Pseudomonas while the remaining two were confirmed under genus Staphylococcus. Results of gram-staining, motility and selected biochemical and physiological tests supported the identity of the four isolates. Pseudomonas isolates were found resistant to 30 µg tetracycline while Staphylococcus isolates were intermediate to susceptible on the said antibiotics. Unhygienic preparation of the smoked fish could be the possible reason for the product contamination. Keywords: Smoked fish, phenotypic characterization, biochemical test, physiological test, antibiotic susceptibilit

Copper sulfate-resistant bacteria isolated from Blue Soil Hills, Sagada, Mountain Province, Philippines

The objective of this study was to isolate copper sulfate-resistant bacteria in Blue Soil Hills, Sagada, Mountain, Province, Philippines. Three distinct bacterial colonies were isolated from the soil sample. The isolates were different from one another based upon colonial characteristics and growth patterns in solid and liquid medium. All three bacteria were gram-positive and were able to grow in medium supplemented with various concentrations of copper sulfate. More luxurious growth was observed in medium with highest supplementation (333.33 ppm). The isolated bacteria could be potential bioremediation agents in soil and water heavily contaminated with copper.&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords: Copper sulfate, bioremediation, Blue Soil Hills, Sagada, Mountain Provinc

Shared Genetic Factors of Anxiety and Depression Symptoms in a Brazilian Family-Based Cohort, the Baependi Heart Study

To investigate the phenotypic and genetic overlap between anxiety and depression symptoms in an admixed population from extended family pedigrees. Participants (n = 1,375) were recruited from a cohort of 93 families (mean age±SD 42±16.3, 57% female) in the rural town of Baependi, Brazil. The Hospital Anxiety and Depression Scale (HADS) was used to assess depression and anxiety symptoms. Heritability estimates were obtained by an adjusted variance component model. Bivariate analyses were performed to obtain the partition of the covariance of anxiety and depression into genetic and environmental components, and to calculate the genetic contribution modulating both sets of symptoms. Anxiety and depression scores were 7.49±4.01 and 5.70±3.82, respectively. Mean scores were affected by age and were significantly higher in women. Heritability for depression and anxiety, corrected for age and sex, were 0.30 and 0.32, respectively. Significant genetic correlations (ρg = 0.81) were found between anxiety and depression scores; thus, nearly 66% of the total genetic variance in one set of symptoms was shared with the other set. Our results provided strong evidence for a genetic overlap between anxiety and depression symptoms, which has relevance for our understanding of the biological basis of these constructs and could be exploited in genome-wide association studies

Heritability of semantic verbal fluency task using time-interval analysis

Individual variability in word generation is a product of genetic and environmental influences. The genetic effects on semantic verbal fluency were estimated in 1,735 participants from the Brazilian Baependi Heart Study. The numbers of exemplars produced in 60 s were broken down into time quartiles because of the involvement of different cognitive processes—predominantly automatic at the beginning, controlled/executive at the end. Heritability in the unadjusted model for the 60-s measure was 0.32. The best-fit model contained age, sex, years of schooling, and time of day as covariates, giving a heritability of 0.21. Schooling had the highest moderating effect. The highest heritability (0.17) was observed in the first quartile, decreasing to 0.09, 0.12, and 0.0003 in the following ones. Heritability for average production starting point (intercept) was 0.18, indicating genetic influences for automatic cognitive processes. Production decay (slope), indicative of controlled processes, was not significant. The genetic influence on different quartiles of the semantic verbal fluency test could potentially be exploited in clinical practice and genome-wide association studies